Histidine detection and estimation in urine

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Abstract
The application of present colorimetric methods for histidine detection and their difficulties are described. A simple and rapid quantitative method for histidine estimation in urine by a modification of Knoop''s bromine reaction is given. The specificity of the bromine test is discussed. When this reaction is applied to normal urine a color is produced which corresponds to an avg. of 20 mg./100 ml. histidine mono-hydrochloride. The range of normal values lies between 2 mg./100 ml. and 80 mg./lOO ml. A higher excretion value in the urines of melancholic patients, as claimed by Schim-melpfeng, could not be confirmed. Pregnancy urines usually show high histidine values when considered in relation to the urea excretion and to the sp. gr. of the urine.

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