The Use of Physiological Characterizations in the Classification of Five Species of Acanthamoeba

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Abstract
Physiological tests were devised and used to compare 15 strains representing 5 spp. of soil and freshwater amoebae assigned to the genus Acanthamoeba [A. castellanii, A. polyphaga, A. rhysodes, A. palestinensis and A. astronyxis]. The tests gave an acceptable level of reliability and the pattern of responses was not affected by the differing growth rates of the organisms. There was some degree of overlap between the strains, as shown by the high level of inter-species similarities in relation to those between strains of the same species. Previous classifications of Acanthamoeba based solely on morphological criteria did not adequately reflect the diversity of these amoebae.

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