Expression of connective tissue growth factor (CTGF) mRNA in plaques of human anterior subcapsular cataracts and membranes of posterior capsule opacification
- 30 August 2000
- journal article
- Published by Taylor & Francis in Current Eye Research
- Vol. 21 (2) , 627-636
- https://doi.org/10.1076/0271-3683(200008)21:2;1-v;ft627
Abstract
Purpose. To investigate the correlation between connective tissue growth factor (CTGF) mRNA expression and immunohistochemical characteristics of anterior subcapsular cataract (ASC) formation as well as posterior capsule opacification (PCO) development (expression of type I collagen, a-smooth muscle actin and tenascin) under in vivo and under in vitro conditions in human and porcine lens epithelial cells. Methods. CTGF mRNA expression was investigated using in situ hybridization and RT-PCR. Expression of type I collagen, a-smooth muscle actin and tenascin was detected by immunohistochemical staining. Results. CTGF mRNA was expressed in human cataractous plaques of ASC and human PCO membranes, and appeared simultanously with the expression of type I collagen, a-smooth muscle actin and tenascin. Conclusion. The predominant expression of CTGF mRNA in human ASC and human PCO membranes suggests a significant role of CTGF in the pathological course of these ocular disorders.This publication has 0 references indexed in Scilit: