Involvement of Rho/ROCK signalling in small cell lung cancer migration through human brain microvascular endothelial cells
Open Access
- 30 June 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (17) , 4252-4260
- https://doi.org/10.1016/j.febslet.2006.06.056
Abstract
Small cell lung cancer (SCLC) cells migration across human brain microvascular endothelial cells (HBMECs) is an essential step of brain metastases. Here we investigated signalling pathways in HBMECs contributing to the process. Inhibition of endothelial Rho kinase (ROCK) with Y27632 and overexpression of ROCK dominant‐negative mutant prevented SCLC cells, NCI‐H209, transendothelial migration and the concomitant changes of tight junction. Conversely, inhibition of phosphatidylinositol 3‐kinase (PI3K) and protein kinase C (PKC) had no effects. Furthermore, endothelial RhoA protein was activated during NCI‐H209 cells transendothelial migration. Rho/ROCK participated in NCI‐H209 cells transendothelial migration through regulating actin cytoskeleton reorganization. These results suggested that Rho/ROCK was required for SCLC cells transendothelial migration.Keywords
This publication has 20 references indexed in Scilit:
- Small-cell lung cancerThe Lancet, 2005
- Cryptococcal Yeast Cells Invade the Central Nervous System via Transcellular Penetration of the Blood-Brain BarrierInfection and Immunity, 2004
- Role of Phosphatidylinositol 3-Kinase in Oxidative Stress-induced Disruption of Tight JunctionsJournal of Biological Chemistry, 2003
- Potential role of MCP-1 in endothelial cell tight junction `opening': signaling via Rho and Rho kinaseJournal of Cell Science, 2003
- Regulation of tight junctions and loss of barrier function in pathophysiologyThe International Journal of Biochemistry & Cell Biology, 2003
- Vascular Endothelial Growth Factor Modulates the Transendothelial Migration of MDA-MB-231 Breast Cancer Cells through Regulation of Brain Microvascular Endothelial Cell PermeabilityJournal of Biological Chemistry, 2003
- Rho GTPases in cell biologyNature, 2002
- Isolation and functional characterization of the actin‐binding region in the tight junction protein ZO‐1The FASEB Journal, 2002
- Signaling from Rho to the Actin Cytoskeleton Through Protein Kinases ROCK and LIM-kinaseScience, 1999
- Phosphorylation and Activation of Myosin by Rho-associated Kinase (Rho-kinase)Journal of Biological Chemistry, 1996