Vitamin K‐dependent carboxylase: Synthesis of an inhibitor of the glutamyl binding site

Abstract
Liver microsomes contain a vitamin K and O2‐dependent carboxylase that converts peptide‐bound glutamyl residues to γ‐carboxyglutamyl residues. The peptide Boc‐O‐phospho—Ser‐O‐phospho—Ser—Leu‐OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent‐solubilized preparation of the carboxylase and is an apparent competitive inhibitor of the carboxylation of Phe—Leu—Glu—Glu—Leu.

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