Growth and Cell Survival Following Treatment with Estramustine, Nor-Nitrogen Mustard, Estradiol and Testosterone of a Human Prostatic Cancer Cell Line (DU 145)

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Abstract
Estramustine at concentrations ranging from 3-40 .times. 10-6 M inhibited the cell growth and clonogenic survival of a human prostatic carcinoma cell line (DU 145). This cell line was unresponsive to estradiol and testosterone at concentrations ranging from 10-9 M to 5 .times. 10-5 M. Metabolism studies with estramustine showed that only a low percentage of the ester linkage was cleaved during the exposure period. This small amount of metabolism could possibly lead to the release of nor-nitrogen mustard, which was found not to be as inhibitory as estramustine in this cell line. Apparently, estramustine per se causes the cell death of hormone unresponsive human prostatic carcinoma cells in cell culture.