The Induced Resistance Response of Carrot Root Slices to Heat-killed Conidia and Cell-free Germination Fluid of Botrytis cinerea Pers. ex Pers.
- 1 June 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 49 (6) , 859-872
- https://doi.org/10.1093/oxfordjournals.aob.a086312
Abstract
Sixty-eight per cent of nuclei in the cells of the upper four layers of carrot slices treated with heat-killed conidia of Botrytis cinerea for 6 h followed by inoculation with live spores for 18 h, migrated to the cell face nearest to the treated surface, compared with 46 per cent in cells of control slices showing a wound-healing response only. Nucleolar volumes in the surface cell layers of control slices increased from a mean of 1.0 μm3 to 3.8 μm3 over 24 h, and in ‘induced’ slices to 7.28 μm3. Using a 40 min pulse of [5−3H]uracil, there was an increase within 15 h of slicing in the number of labelled nuclei in cells from control slices undergoing healing. Within 8 h after treatment of slice surfaces with heat-killed conidia, there was an accelerated incorporation of label into ‘nuclear’ RNA. Slices from roots cold-stored for 12 months failed to show an induction response and nucleolar volumes did not increase more than in control slices. These results are discussed in relation to active defence mechanisms in plant tissue.Keywords
This publication has 1 reference indexed in Scilit:
- Wound-Healing in Higher PlantsPublished by Elsevier ,1970