Isolation and characterization of genomic moose DNA clones containing sequences homologous to tRNAs and 5S rRNA
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (14) , 4809-4821
- https://doi.org/10.1093/nar/11.14.4809
Abstract
We have cloned and characterized three fragments of Balb/c mouse DNA which hybridize to mouse cell tRNAs. Fractionation of the tRNAs which hybridize to these clones reveals that two of the clones, λMt-4A and λMt-6A hybridize to only one or two tRNAs, while one clone, λMt-4B, hybridizes to at least seven tRNAs. Two of the tRNAs were identified as and , and others have been identified as tRNAs which are selectively encapsidated into virions of rourine leukemia virus and avian reticuloendotheliosis virus. The DNA sequences of putative genes for and , plus flanking regions, were determined. A clone of Balb/c mouse DNA which selectively hybridized to 5S rRNA was also isolated and partially characterized.Keywords
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