Aneuploidy induced by cholral hydrate detected in human lymphocytes with the Y97 probe
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 5 (6) , 591-592
- https://doi.org/10.1093/mutage/5.6.591
Abstract
Chloral hydrate (CH) has been assayed for its ability to induce chromosome number variation in human lymphocytes in culture. Aneupolidy induction has been detected by means of in situ hybridization on interphase nuclei with a chromosome Y-specific DNA probe. A dose-dependent increase in the number of hyperdiploid nuclei was found at CH concentrations ranging from 250 to 750 .mu.g/ml. These results represent a further contribution to the validation of the method which gave a positive responses with drugs characterized by different mechanisms of action and therefore may be of general use in detecting aneuploidy in mammalian cells.This publication has 6 references indexed in Scilit:
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