AN AUTOMATED RETICULOCYTE COUNTING METHOD - PRELIMINARY-OBSERVATIONS

  • 1 April 1989
    • journal article
    • research article
    • Vol. 46  (2) , 157-160
Abstract
We evaluated the counting of reticulocytes in the peripheral blood with a newly developed flow cytometer type of automated counter that performs a single test within 60 s. The volume of sample needed is 100 .mu.l and the cells are stained with auramine-O in the counter. The mean within-run reproducibility was 4.66% (CV, n = 50), and dilution of blood gave highly linear results with an r value of 0.996. Correlation was good between manual reticulocyte counts and those performed with the counter (r = 0.893). Samples with large numbers of leucocytes, erythrocytes, or platelets did not interfere with the automated reticulocyte counting, and provided accurate and precise data.

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