A simple technique for selective staining of neurosecretory products in epoxy sections with paraldehyde fuchsin

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Abstract
A technique for correlative light and electron microscopy of neurosecretory cells (NSC) is described. Neurosecretory material is selectively stained in 0.5-μm epoxy sections with paraldehyde fuchsin (PAF) after partial resin removal with sodium methylate in methanol and oxidation in peracetic acid. Adjacent 'thin' sections permit electron microscopy of the same cells. The organelles responsible for the affinity of NSC for PAF are in part neurosecretory granules and in part lysosomes. The technique reveals great ultrastructural differences between NSC which appear similar in the light microscope.

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