A New Method of Antibody Elution From Red Blood Cells

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Abstract
A method of antibody elution from red blood cells using xylene was described. It can be completed in 15 min after cell washing and requires no special equipment. For warm antibodies, the eluates prepared were more potent than those prepared by Rubin''s ether method. It was as effective as Landsteiner and Miller''s heat elution method in investigations of ABO hemolytic disease of the newborn. The clinical application of this method in the investigation of hemolytic disease of the newborn, delayed transfusion reaction and autoimmune hemolytic anemia was described.

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