Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNA

1 May 1975

journal article
research article
Published by Springer Nature in Nature

Vol. 255 (5503) , 28-33
https://doi.org/10.1038/255028a0

Abstract

Messenger RNAs from mouse myeloma cells contain N6-methyl adenosine and novel 5′ termini having 7-methylguanosine in a 5′, 5′triphosphate linkage with ribose-methylated nucleosides. Ten common 5′-terminal sequences of the forms m⁷G^5′ppp^5′NmpNp and m⁷G^5′ppp^5′NmpNmpNp are reported. Structures like this may be a general feature of mRN A in eukaryotes.

Keywords

This publication has 35 references indexed in Scilit:

A blocked structure at the 5′ terminus of mRNA from cytoplasmic polyhedrosis virus
Nature, 1975
A modified structure at the 5′‐terminus of mRNA of vaccinia virus
FEBS Letters, 1975
Sequences containing methylated nucleotides at the 5′ termini of messenger RNAs: Possible implications for processing
Published by Elsevier ,1974
Eukaryotic Messenger RNA
Annual Review of Biochemistry, 1974
Existence of methylated messenger RNA in mouse L cells
Cell, 1974
Analysis of the Methylation Sites in Yeast Ribosomal RNA
European Journal of Biochemistry, 1973
Fusion of Two Immunoglobulin-producing Myeloma Cells
Nature, 1973
Fractionation of Membrane‐Bound Polysomes, Free Polysomes, and Nuclei from Tissue‐Cultured Cells
European Journal of Biochemistry, 1973
A new method using ‘proteinase K’ to prevent mRNA degradation during isolation from HeLa cells
Biochemical and Biophysical Research Communications, 1971
SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS
The Journal of Experimental Medicine, 1970