[LeuB24]- and [LeuB25]Insulins are not Antagonists of Lipogenesis in Adipocytes
- 1 January 1982
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 363 (1) , 187-192
- https://doi.org/10.1515/bchm2.1982.363.1.187
Abstract
Semisynthetic human [LeuB24]- and [LeuB25]insulins were investigated to determine whether they show antagonistic properties towards insulin-stimulated lipogenesis in isolated [rat] fat cells. In contrast to other reports, only an additive agonistic effect could be detected when constant concentrations (e.g. 0.3 ng/ml) of the analogs were mixed with varying concentrations of insulin, or when constant concentrations (e.g. 0.3 ng/ml, 0.6 ng/ml) of inuslin were mixed with varying concentrations of the analogs. Similar results were obtained with mixtures of insulin and NA2-acetyl- or NA2-propionyl-des-GlyA1-insulins. These results do not support the contention that a diabetic state could be caused by either of these mutant human insulins.This publication has 4 references indexed in Scilit:
- Impaired negative cooperativity of the semisynthetic analogues human [LeuB24]- and [LeuB25]-insulinsBiochemical and Biophysical Research Communications, 1981
- Characterization of a mutant human insulin species.Journal of Biological Chemistry, 1980
- Semisynthesis and biological activity of porcine [LeuB24]insulin and [LeuB25]insulin.Proceedings of the National Academy of Sciences, 1980
- A structurally abnormal insulin causing human diabetesNature, 1979