Evidence for stacking interactions between 5-mercurated polyuridylic acid and HIV-1 p7 nucleocapsid protein obtained by phosphorescence and optically detected magnetic resonance (ODMR)
- 9 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 328 (1) , 45-48
- https://doi.org/10.1016/0014-5793(93)80962-t
Abstract
The photoexcited triplet state of Trp-37 in the C-terminal zinc finger of the HIV-1 p7 nucleocapsid protein was used as a probe of p7 interactions with the heavy atom-derivatized RNA homopolymer, poly-5-mercuriuridylic acid (5-HgU). Binding of p7 to 5-HgU (Hg blocked with 2-mercaptoethanol) produces an external heavy atom effect (HAE) on Trp-37 characterized by fluorescence quenching, reduction of the phosphorescence lifetime by three orders of magnitude, and the appearance of the D + E phosphorescence-detected ODMR signal, absent in unperturbed Trp, but induced by a HAE. The details of the HAE are consistent with out-of-plane van der Waals contact of Hg with the indole chromophore of Trp-37. Steric requirements suggest further that the Trp-RNA contact occurs via an aromatic stacking interaction.Keywords
This publication has 19 references indexed in Scilit:
- Zinc‐ and sequence‐dependent binding to nucleic acids by the N‐terminal zinc finger of the HIV‐1 nucleocapsid protein: NMR structure of the complex with the Psi‐site analog, dACGCCProtein Science, 1993
- Nucleocapsid zinc fingers detected in retroviruses: EXAFS studies of intact viruses and the solution‐state structure of the nucleocapsid protein from HIV‐1Protein Science, 1992
- Retroviral nucleocapsid protein-metal ion interactions: folding and sequence variants.Proceedings of the National Academy of Sciences, 1990
- Triplet state properties of tryptophan residues in complexes of mutated Escherichia coli single-stranded DNA binding proteins with single-stranded polynucleotidesBiophysical Journal, 1989
- Point mutants of Moloney murine leukemia virus that fail to package viral RNA: evidence for specific RNA recognition by a "zinc finger-like" protein sequence.Proceedings of the National Academy of Sciences, 1988
- P10, A low molecular weight single-stranded nucleic acid-binding protein of murine leukemia retroviruses, shows stacking interactions of its single tryptophan residue with nucleotide basesBiochemistry, 1988
- Potential Metal-Binding Domains in Nucleic Acid Binding ProteinsScience, 1986
- It is Rous Sarcoma virus protein P12 and not P19 that binds tightly to Rous Sarcoma virus RNAJournal of Molecular Biology, 1984
- Techniques, Theory, and Biological Applications of Optically Detected Magnetic Resonance (ODMR)Published by Springer Nature ,1984
- Assembly of Type C Oncornaviruses: A ModelScience, 1978