A COMPARISON OF METHODS FOR DECALCIFYING BONE
Open Access
- 1 September 1962
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 10 (5) , 560-563
- https://doi.org/10.1177/10.5.560
Abstract
Ground fresh compact beef bone was decalcified in varying strengths of acids and in the sodium salts of EDTA. Samplings of the solutes were measured for nitrogen and calcium at time intervals. It was found on acid decalcification that measurable quantities of nitrogen were removed with the weakest acid used and for the shortest time interval taken. No measurable quantities of nitrogen were removed by the chelating agent when it was maintained at pH 7.4. The rate of nitrogen removal was strongly retarded by prior fixation of the bone with formalin.This publication has 0 references indexed in Scilit: