Identification of Lactate Dehydrogenase Isoenzymes by Rapid Kinetics
- 1 July 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (7) , 1761-1765
- https://doi.org/10.1073/pnas.69.7.1761
Abstract
Stopped-flow kinetics indicate that human and chicken heart-type(4) lactate dehydrogenases (LDH) become inhibited as DPNH is oxidized in the presence of high concentrations of pyruvate. This inhibition is much less marked with the human and chicken muscle-type(4) enzymes. The initial rates and the difference in inhibition between the two types of enzyme have made it possible to determine the amount of, as well as the ratio between, the two types of LDH that are present in a given sample by a single kinetic assay. The stopped-flow kinetic method has been used to analyze amounts of LDH isoenzyme in different tissues, as well as in serum.Keywords
This publication has 17 references indexed in Scilit:
- The formation of ternary complexes by diphosphopyridine nucleotide-dependent dehydrogenasesArchives of Biochemistry and Biophysics, 1971
- SIGNIFICANCE OF SUBSTRATE INHIBITION OF DEHYDROGENASES*Annals of the New York Academy of Sciences, 1968
- The kinetics of the reversible inhibition of heart lactate dehydrogenase through the formation of the enzyme–oxidized nicotinamide–adenine dinucleotide–pyruvate compoundBiochemical Journal, 1968
- COMPARATIVE ENZYMOLOGY OF LACTIC DEHYDROGENASES .3. PROPERTIES OF H4 AND M4 ENZYMES FROM A NUMBER OF VERTEBRATES1967
- LACTATE DEHYDROGENASE--STRUCTURE AND FUNCTION.1964
- THE COMPARATIVE ENZYMOLOGY OF LACTIC DEHYDROGENASES. I. PROPERTIES OF THE CRYSTALLINE BEEF AND CHICKEN ENZYMES.1964
- The Comparative Enzymology of Lactic Dehydrogenases. II. Properties of the Crystalline HM3 Hybrid from Chicken Muscle and of H2M2 Hybrid and H4 Enzyme from Chicken Liver*Biochemistry, 1964
- SERUM LACTIC DEHYDROGENASE ISOZYME PATTERNS IN CARDIOVASCULAR + OTHER DISEASES WITH PARTICULAR REFERENCE TO ACUTE MYOCARDIAL INFARCTION1964
- LACTIC DEHYDROGENASE ISOZYMES AND THEIR DISTRIBUTION IN NORMAL TISSUES AND PLASMA AND IN DISEASE STATES*Annals of the New York Academy of Sciences, 1961
- OBSERVATIONS ON THE HETEROGENEITY OF MALIC AND LACTIC DEHYDROGENASE IN HUMAN SERUM AND RED BLOOD CELLS1Journal of Clinical Investigation, 1958