Bordetellae and Charcoal Horse Blood Agar: Inactivation of Antibiotics in Agar during Prolonged Incubation for Susceptibility Testing

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Abstract
We examined the degree of inactivation of 22 antibiotics caused by prolonged incubation at 36 °C of agar plates during agar dilution susceptibility testing of Bordetellae. Fresh antibiotic-containing plates of charcoal horse blood agar and plates which had been held at 36 °C for 2 or 3 days prior to inoculation were inoculated with strains of Bordetella pertussis and Bordetella parapertussis and incubated for 2 days. Then the MICs were compared. Most antibiotics showed a loss of activity of up to four dilution steps after prolonged incubation. Further studies with other slow-growing organisms and other complex media are needed.

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