Two color hybridization analysis using high density oligonucleotide arrays and energy transfer dyes
Open Access
- 1 August 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 26 (16) , 3865-3866
- https://doi.org/10.1093/nar/26.16.3865
Abstract
High density oligonucleotide arrays (DNA chips) have been used in two color mutational analysis of the 3.43 kb exon 11 of the hereditary breast and ovarian cancer gene BRCA1. Two color analysis allows competitive hybridization between a reference standard and an unknown sample, improving the performance of the assay. Fluorescein and phycoerythrin dyes were previously used due to their compatibility with a single line 488 nm excitation source. Here we show that an alternative dye combination, containing the energy transfer dye system phycoerythrin·cy5 along with phycoerythrin, provides more evenly matched signal intensities and decreased spectral overlap between the two fluorophores, while maintaining compatibility with a 488 nm excitation source.Keywords
This publication has 3 references indexed in Scilit:
- Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysisNature Genetics, 1996
- Accessing Genetic Information with High-Density DNA ArraysScience, 1996
- Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.Proceedings of the National Academy of Sciences, 1995