INHIBITION OF CELL MIGRATIONS BY THE LINOLEIC-ACID OXYGENATION PRODUCT HYDROXY 10E,12Z OCTADECADIENOIC ACID (9-HODE)

  • 1 January 1988
    • journal article
    • research article
    • Vol. 47  (12) , K17-K21
Abstract
The linoleic acid metabolite 9S-HODE was prepared by means of tomato fruit lipoxygenase and purified by high-performance liquid chromatography (HPLC) to a high steric purity as judged by chiral-phase HPLC. 9S-HODE caused in the concentration range between 0.01 and 1 .mu.M a strong dose-dependent inhibition of the migration of both cultured porcine aortic endothelial cells and of phytohaemagglutinin-stimulated human mononuclear cells. The effect was not observed with another polyenoic fatty acid metabolite, 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HPETE). The results are discussed in the light of other biological actions of 9-HODE recently described.