The Effects of Glibenclamide and its Non-Sulfonylurea Analogue HB 699 on the Sodium Content of Rat Pancreatic Islets
- 1 December 1989
- journal article
- research article
- Published by Georg Thieme Verlag KG in Experimental and Clinical Endocrinology & Diabetes
- Vol. 93 (02/03) , 299-306
- https://doi.org/10.1055/s-0029-1210872
Abstract
Sodium contents were determined in rat pancreatic islets using integrating flame photometry. Whereas the sodium content decreased in the presence of glucose, it increased when 0.1—100 μmol/1 glibenclamide was added to a medium containing 3 mmol/1 glucose. The complexity of the glibenclamide action became evident with its reversal after removal of extracellular Ca2+ and the observation that the sulfonylurea counteracted the increase of sodium obtained after removal of K+. The effects of glibenclamide were mimicked by 1 mmol/1 of its non-sulfonylurea analogue HB 699 with the exception that the latter compound being without suppressive action on the sodium content in medium deprived of Ca2+- Also exposure to 1 mmol/1 sulfadiazine resulted in a Ca2+-dependent increase of sodium. The results suggest a role for sodium in amplifying the secretory response to the increased entry of Ca2+ obtained with the depolarisation of the β-cells with glibenclamide or HB 699.Keywords
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