Thyrotropin‐releasing hormone enhances motor neuron–evoked contractions of cultured human muscle
- 1 December 1985
- journal article
- research article
- Published by Wiley in Annals of Neurology
- Vol. 18 (6) , 716-719
- https://doi.org/10.1002/ana.410180615
Abstract
Thyrotropin-releasing hormone (TRH), 1 to 2.7 mM, introduced to the medium of monolayer-cultured human muscle innervated by cocultured fetal rat spinal cord with dorsal root ganglia, increased muscle fiber contractions 4.9 times (p < 0.005). Doses higher than 5 mM, after increasing contractions for a few seconds, caused complete inhibition of contraction (autorefractoriness). Neither aneurally cultured human muscle nor innervated cultured human muscle blocked by 1 mM d-tubocurarine was excited by TRH. Thus, in this system TRH appeared to excite muscle contractions through direct or indirect action on motor neurons.This publication has 7 references indexed in Scilit:
- Acetylcholine receptors and acetylcholinesterase accumulate at the nerve-muscle contacts ofde novo grown human monolayer muscle cocultured with fetal rat spinal cordExperimental Neurology, 1985
- Thyrotropin‐Releasing Hormone Enhances Choline Acetyltransferase and Creatine Kinase in Cultured Spinal Ventral Horn NeuronsJournal of Neurochemistry, 1984
- EFFECT ON WEAKNESS AND SPASTICITY IN AMYOTROPHIC LATERAL SCLEROSIS OF THYROTROPIN-RELEASING HORMONEThe Lancet, 1983
- ACTION OF THYROTROPIN-RELEASING-HORMONE, SUBSTANCE-P AND RELATED PEPTIDES ON FROG SPINAL MOTONEURONS1978
- Excitatory action of TRH on spinal motoneuronesNature, 1977
- Thyrotropin releasing hormone (TRH)-containing nerve terminals in certain brain stem nuclei and in the spinal cordNeuroscience Letters, 1975
- A new program for investigating adult human skeletal muscle grown aneurally in tissue cultureNeurology, 1975