Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae
- 1 April 1992
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 21 (4-5) , 285-289
- https://doi.org/10.1007/bf00351684
Abstract
A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 μm DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.Keywords
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