Isozyme differentiation among three pathotypes of the entomogenous fungus Nomuraea rileyi

Abstract
The zymogram technique was applied to 3 pathotypes of the entomopathogenic fungus N. rileyi. Isozyme profiles of isolates from Heliothis zea, Pseudoplusia includens and Anticarsia gemmatalis were compared for 17 enzymes of known metabolic function. Electrophoretic data supported the taxonomic differences inferred for the 3 pathotypes based on host specificity. The isolate from A. gemmatalis was consistently the most distinct. This study demonstrates that isozyme analysis may be used to distinguish closely related fungal isolates of N. rileyi.