Degradation of side-chains of N-(2-hydroxypropyl)methacrylamide copolymers by lysosomal thiol-proteinases
- 1 December 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (12) , 1041-1046
- https://doi.org/10.1007/bf01122173
Abstract
N-(2-Hydroxypropyl)methacrylamide copolymers bearing oligopeptide side-chains terminating in p-nitroaniline (NAp) [.alpha. potential drug delivery system] were incubated with rat liver lysosomal enzymes in the presence of the thiol glutathione, and the rate of Nap release was measured. Twelve of the 16 side-chains investigated were hydrolyzed to release NAp and in all but 1 case degradation was partially or totally inhibited by leupeptin. The effect of substrate concentration on the degradation of the most readily cleaved side-chain, -Ala-Gly-Val-Phe-NAp, was measured.This publication has 8 references indexed in Scilit:
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