Neutral Lipid Class Fractionation and Further Separation of Simple Neutral Glycolipids by OPTLC

Publisher Website
Google Scholar
Abstract
In lipid preparation from biological sources extraction and chromatographic methods are widely used. The use of the OPTLC method is introduced in the present paper. The method is suitable for the class separation of the neutral fraction of a total lipid extract with a single isocratic run. With a step or an exponential gradient the simple neutral glycolipids can be separated. Either elution can be performed on a 10 × 20 cm plate on 12 parallel samples. The chromatograms were evaluated by densitometric scanning after staining with orcinol-H2SO4 reagent.