DIURNAL-VARIATION OF CIRCULATING HUMAN MYELOID PROGENITOR CELLS

Google Scholar
  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (7) , 954-960
Abstract
Human circulating myeloid progenitor cells (CFUC) exhibition of diurnal variation was investigated. Peripheral venous blood samples were drawn every 3 h from each of 9 healthy male volunteers. Blood mononuclear cells were harvested by the Ficoll-Hypaque method. CFUC [granulocytic progenitor cells] content of blood was determined by the soft agar cloning assay. Human placental conditioned medium (HPCM) was used as colony stimulating factor. A 24-h cyclic variation of colonies/105 mononuclear cells plated, with a maximum at 0900 h was found (P < 0.01). Analysis of the colonies by in situ staining indicated that this variation was mostly due to colonies composed of eosinophilic cells (representative of CFUeos). Neutrophil/macrophage colonies (representative of CFUG,M) exhibited a trend toward a 0900-h maximum, but this variation was not statistically significant. The time of blood collection should probably be standardized in experiments measuring human peripheral blood CFUC.