Histochemical detection of DNA strand scissions in mammalian cells by nick translation
- 30 April 1985
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 9 (5) , 471-477
- https://doi.org/10.1016/0309-1651(85)90155-9
Abstract
A method to visualize in situ of single strand scissions of DNA in fixed mammalian cells has been developed. Using the nuclear nick translation with biotin-labeled dUTP followed by binding to avidin-biotin-peroxydase complex, the nuclei of HeLa cells which had been treated with a DNA-damaging antibiotic bleomycin were specifically stained, implicating that the histochemical detection of single strand scissions (nicks) of DNA in fixed cells was completed without destroying the morphology, and without using autoradiography.This publication has 5 references indexed in Scilit:
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