Influence of a Cartilage Bone Marrow Extract on Mucopolysaccharide Metabolism of Cells Cultured in vitro

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Abstract
Treatment of fibroblast cultures with cartilage extracts or cartilage bone marrow extracts has an influence on the mucopolysaccharides produced by the cells and released into the culture medium, mucopolysaccharides being determined as measurement of turbidity. By separation of cartilage extract on Sephadex G-25 3 fractions were obtained: a high molecular fraction, a fraction containing free amino acids and nucleotides, a cresol containing fraction. The 1st (high molecular) fraction is responsible for the increase in turbidity of the medium of fibroblast cultures; the 2nd fraction induces an increased sulfate incorporation into cartilage in vitro.

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