Induction of proliferation and differentiation of neoplastic B cells by anti-immunoglobulin and T-cell factors.

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Abstract
Sepharose-bound anti-immunoglobulins, which are potent mitogens for normal adult B cells, are not mitogenic for tumor cells freshly isolated from mice carrying the B-cell leukemia BCL1. After 4 or more days of in vitro cultivation, BCL1 cells can be stimulated to divide by anti-.mu. or anti-.delta. antibodies. In vitro cultivation of BCL1 cells apparently results in their differentiation into more mature cells which can be triggered to proliferate by their interaction with anti-Ig antibodies. Addition of T-cell helper factors to anti-Ig-treated BCL1 cells results in their differentiation into Ig-secreting cells. Surface Ig molecules on BCL1 cells are capable of delivering an activation signal to the cells, but the cells require a second signal from T cells for induction of Ig secretion.