The Effect of Pressure on the Retention Time and the Retention Volume of an Inert Compound In Liquid Chromatography

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Abstract
The effect of pressure on the carrier liquid flow-rate, retention volumes and retention times in liquid chromatography have been investigated. These effects result from the variation of the density and viscosity of liquids with pressure, from the deviation from Darcy's law at large flow velocities, and from the deformation of column wall and packing under pressure. It is shown that whereas the liquid compressibility usually gives the major contribution to the variation of retention volume which may reach 10% for ΔP = 1000 bars, the variation of viscosity with pressure will result in marked deviation from the expected variation of the retention time in proportion to the inverse of the pressure drop. Deviation as large as 12% may occur for an inlet pressure of 200 bars.

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