The attachment of IgG to cell components: a reconsideration of Brambell’s receptor hypothesis of protein transmission
- 17 September 1974
- journal article
- Published by The Royal Society in Proceedings of the Royal Society of London. B. Biological Sciences
- Vol. 187 (1087) , 209-219
- https://doi.org/10.1098/rspb.1974.0070
Abstract
Nuclear, mitochondrial and microsomal fractions were prepared from rabbit yolk-sac splanchnopleur and rabbit chorio-allantoic placenta, and tested in vivo and in vitro for attachment of labelled rabbit and bovine IgG. Similar fractions were prepared from small intestinal scrapings of neonatal rats, and tested in vivo and in vitro for attachment of labelled rat and sheep IgG. In all cases the attachment of the homologous and heterologous proteins was substantially equal. Digestion experiments on the labelled organelle pellets of rabbit yolk-sac were carried out with the proteases of the macerated tissue (the cell sap or final supernatant) at optimum pH. The nuclear pellet gave rise to a higher proportion of breakdown products derived from bovine than from rabbit IgG, showing the existence of a mechanism whereby selection between the two proteins might be brought about.Keywords
This publication has 12 references indexed in Scilit:
- INTESTINAL TRANSPORT OF ANTIBODIES IN THE NEWBORN RATThe Journal of cell biology, 1973
- The mechanism of intestinal uptake and transcellular transport of IgG in the neonatal ratJournal of Clinical Investigation, 1972
- Proteolytic enzymes of the yolk-sac splanchnopleurBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- Intrinsic Factor-mediated Attachment of Vitamin B12 to Brush Borders and Microvillous Membranes of Hamster Intestine*Journal of Clinical Investigation, 1967
- THE TRANSMISSION OF IMMUNITY FROM MOTHER TO YOUNG AND THE CATABOLISM OF IMMUNOGLOBULINSThe Lancet, 1966
- Labelling of human fibrinogen with 131I by electrolytic iodinationBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- The LysosomeScientific American, 1963
- The occurrence of macroglobulin antibodies in maternal and foetal sera of rabbits as determined by gradient centrifugationProceedings of the Royal Society of London. B. Biological Sciences, 1962
- Protein selection in the yolk-sac splanchnopleur of the rabbit: the distribution of isotope following injection of 131 I-labelled serum globulin into the uterine cavityProceedings of the Royal Society of London. B. Biological Sciences, 1956
- Prenatal and postnatal transmission of passive immunity to young ratsProceedings of the Royal Society of London. B. Biological Sciences, 1955