Effect of a pesticide, pentachlorophenol (PCP) on soil microflora. II. Effect of PCP on bacterial flora in soil percolated with glycine or water.

Publisher Website
Google Scholar
Abstract
The soil bacterial floras were studied in glycine- and water-percolated soils with or without PCP treatment. Many bacterial strains isolated from the percolated soils with PCP treatment died after purification, while those from the percolated soils without PCP did not die. Of the living strains, the bacterial flora in water-percolated soil with PCP treatment was composed of one specific bacterial group: gram-negative, coccoidal rod-shaped, polarly flagellated, Kovacs'' oxidase positive, and neither oxidative nor fermentative in Hugh and Leifson''s test. On the other hand, the bacterial flora in water-percolated soil without PCP was composed of several bacterial groups. In the glycine-percolated soil without PCP treatment one group of bacteria predominated: gram-negative, rod-shaped, polarly flagellated, Kovacs'' oxidse positive, oxidative acid production from glucose in Hugh and Leifson''s test, gelatin-liquefaction negative, nitrate-reduction positive, and alkaline reaction to BCP milk. However, different groups of bacteria were isolated from the glycine-percolated soil treated with PCP. Almost all of the strains isolated from the glycine-percolated soil without PCP treatment readily utilized glycine for their growth, but those isolated from the soil with PCP added utilized glycine scantily for their growth. These results indicate that PCP affects soil bacterial flora and that the bacteria responsible for glycine degradation in the glycine-percolated soil treated with PCP are different from the bacterial flora in soil without PCP, even though the apparent patterns of both glycine-degradation and the increase of bacterial population did not change in the presence of PCP.