PHOTODYNAMIC INACTIVATION OF SYNCHRONIZED HUMAN-CELLS INVITRO IN THE PRESENCE OF HEMATOPORPHYRIN

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (9) , 3735-3737
Abstract
Dose-response curves for inactivation of synchronized cells from the human cell line NHIK 3025 [cervical carcinoma] in the presence of hematoporphyrin and light [a tumor therapy procedure] are shown. Sensitivity toward photodynamic inactivation is increasing as the cells move in the cell cycle from G1 to mid-S. The increase is mostly due to a change in the extent of the shoulder of the dose-response curve at low doses. The quasithreshold dose (Dq) is high in early G1 and much lower in mid-S. No great variations were found in the slope (1/Do) [1/mean lethal dose] of the dose-response curves at high doses. The shape of the dose-response curves indicates a higher capacity for repair of sublethal damage in the early part of the cell cycle than in the later stages.