Some comparative observations on the relative contributions of alternate pathways in the metabolism of glucose by Candida utilis

Publisher Website
Google Scholar
Abstract
C. utilis was grown in batch, chemostat, and continuously synchronized (phased) culture on a N-limited glucose mineral salts medium: P- and C-limited phased cultures were also used. The 14CO2 evolved from [G-1-14C]glucose labeled in the C 1 position and [G-6-14C] was used, as a simple C1/C6 ratio, to observe the relative changes in EMP (Embden-Meyerhof-Parnas) pathway and HMP (hexose monophosphate pathway) contributions during growth of the cultures. The ratio varied during the cell cycle, and changed with growth rate, and with nutrient limitation. The changes generally indicated that the HMP predominated, most notably in the early part of the batch-growth sequence and early in the cell cycle. The overall results reflected the relative merits of the different cultivation techniques for examining microbial metabolism: the advantage of a greater resolution by the synchronized method, based upon the unit performance rather than the randomized mean performance of the cell population, was demonstrated.

This publication has 3 references indexed in Scilit: