Immobilized Enzyme Modules for Use in Analytical Microcalorimetry

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Abstract
Immobilized enzyme modules designed for use in a conduction type analytical microcalorimeter were fabricated from cellulose acetate tubes and from cellulose filter paper. Catalase was bound to the modules after cyanogen bromide activation. Repetitive substrate determinations were made over a range of 0.015–1.2 mg of H2O2/ml of analytical solution. This was possible in spite of qualitative response differences relative to soluble enzyme reactions. The enzyme modules demonstrated excellent long term stability after repeated use and storage promising significant advantage for routine calorimetry using enzyme reagents.