Time-resolved fluoroimmunoassay of pancreatic phospholipase A2
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Medicine
- Vol. 67 (3) , 103-105
- https://doi.org/10.1007/bf01711331
Abstract
We have developed an immunoassay for human pancreatic secretory phospholipase A2 based on time-resolved fluorescence. The labeled antibody technique in combination with the time-resolved fluorometric detection of the Europium label, which essentially eliminates all background fluorescence, resulted in a high sensitivity and a wide linear range. Our assay is a one-incubation, multi-site, solid-phase assay on polystyrene micro-titer strips, even though a polyclonal antibody was used. Increased levels of immunoreactive phospholipase A2 are found by this assay in sera of patients suffering from acute pancreatitis.Keywords
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