The new antibiotic complex A35512 produced by S. candidus was isolated from the filtered fermentation broth. The individual factors A, B, C, E and H were separated and purified by column chromatography. A35512B, the major factor, was isolated as the dihydrochloride salt, a white crystalline compound with an approximate empirical formula of C90H101 N8O39Cl.cntdot.2HCl. The A35512 antibiotics belong to the glycopeptide class of antibiotics and possess high in vitro and in vivo activity against gram-positive bacteria.