CONVENIENT USES OF POLYMERASE CHAIN-REACTION IN ANALYZING RECOMBINANT CDNA CLONES

  • 1 July 1989
    • journal article
    • research article
    • Vol. 7  (7) , 730-+
Abstract
We have used polymerase chain reaction to accelerate our analysis of recombinant .lambda.-cDNA clones. We have amplified the inserts of .lambda.gt10 or .lambda.gt11 recombinants starting with bacteriophage in cored plaques or isolated DNA. The amplifications made with simple or complex oligonucleotide primers have allowed convenient sizing, subcloning and translation of the phage inserts into protein.

This publication has 0 references indexed in Scilit: