Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix
- 1 February 1977
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 74 (1) , 19-26
- https://doi.org/10.1017/s0031182000047491
Abstract
After infection with Eimeria necatrix, chick intestinal cells undergo a profound increase in size. Herien, we describe methods for isolating and purifying infected cells containing second-generation schizonts at Various times post-infection. Our purification procoedures depended upon three fundamental properties of infected cells: namely (1) large size, (2) resistance to shearing and (3) decreased susceptibility to osmotic shock in hypotonic solutions. The availability of simple techniques for isolating infected whole cells should prove valuable in elucidating various intracellular aspects of infection during schizogony.This publication has 3 references indexed in Scilit:
- Induction of host nuclear DNA synthesis in coccidia-infected chicken intestinal cellsInternational Journal for Parasitology, 1974