Structure of the PIIsignal transduction protein ofNeisseria meningitidisat 1.85 Å resolution
Open Access
- 31 May 2006
- journal article
- structural genomics-communications
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Vol. 62 (6) , 494-497
- https://doi.org/10.1107/s1744309106015430
Abstract
The PII signal transduction proteins GlnB and GlnK are implicated in the regulation of nitrogen assimilation in Escherichia coli and other enteric bacteria. PII-like proteins are widely distributed in bacteria, archaea and plants. In contrast to other bacteria, Neisseria are limited to a single PII protein (NMB 1995), which shows a high level of sequence identity to GlnB and GlnK from Escherichia coli (73 and 62%, respectively). The structure of the PII protein from N. meningitidis (serotype B) has been solved by molecular replacement to a resolution of 1.85 Å. Comparison of the structure with those of other PII proteins shows that the overall fold is tightly conserved across the whole population of related proteins, in particular the positions of the residues implicated in ATP binding. It is proposed that the Neisseria PII protein shares functions with GlnB/GlnK of enteric bacteria.Keywords
This publication has 37 references indexed in Scilit:
- Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidisBMC Structural Biology, 2005
- PII signal transduction proteins: sensors of α-ketoglutarate that regulate nitrogen metabolismCurrent Opinion in Microbiology, 2005
- The structures of the PII proteins from the cyanobacteriaSynechococcus sp. PCC 7942 andSynechocystis sp. PCC 6803Acta Crystallographica Section D-Biological Crystallography, 2003
- Multiple sequence alignment with the Clustal series of programsNucleic Acids Research, 2003
- PIISignal Transduction Proteins, Pivotal Players in Microbial Nitrogen ControlMicrobiology and Molecular Biology Reviews, 2001
- Signal transduction to the Azotobacter vinelandii NIFL-NIFA regulatory system is influenced directly by interaction with 2-oxoglutarate and the PII regulatory proteinThe EMBO Journal, 2000
- The Escherichia coli signal transducers PII (GlnB) and GlnK form heterotrimers in vivo : Fine tuning the nitrogen signal cascadeProceedings of the National Academy of Sciences, 2000
- PII signal transduction proteinsTrends in Microbiology, 2000
- Structure/function analysis of the PII signal transduction protein of Escherichia coli: genetic separation of interactions with protein receptorsJournal of Bacteriology, 1997
- Probing interactions of the homotrimeric PII signal transduction protein with its receptors by use of PII heterotrimers formed in vitro from wild-type and mutant subunitsJournal of Bacteriology, 1997