Acid Formation from Penicillin During Enzymatic Inactivation

23 February 1945

journal article
research article
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 101 (2617) , 205
https://doi.org/10.1126/science.101.2617.205

Abstract

A cell-free enzyme soln., presumably penicillinase, was prepd. from an aerobic spore-forming bacterium, apparently Bacillus sub-tilis, which was isolated from a contaminated Penicillium notatum culture. Upon adding 0.03% NaHC03 there was a concomitant evolution of CO2 gas which ceased after 1 hr. A sample removed from the Warburg respirometer showed no penicillin remaining.