Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Clinical and Experimental Hypertension. Part A: Theory and Practice
- Vol. 12 (1) , 83-95
- https://doi.org/10.3109/10641969009074721
Abstract
A microplate-ELISA was established for angiotensin (Ang) I using Ang I -peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3′,5,5′-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.Keywords
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