Application of 13C Nuclear Magnetic Resonance Spectroscopy to Molecular Structural Analyses of Antibody Molecules1
- 1 June 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 105 (6) , 867-869
- https://doi.org/10.1093/oxfordjournals.jbchem.a122769
Abstract
A 13C nuclear magnetic resonance study of a mouse anti-dansyl monoclonal antibody is reported. The antibody molecule was specifically labeled with [1-13C]methionine by growing hybridoma cells in serum-free medium. It was possible to observe all the carbonyl carbon resonances of the antibody. Fab and Fc fragments have been obtained from the antibody and used successfully for the assignment of each of the carbonyl resonances to either the Fab or Fe region. It has been shown that the spectrum of the intact antibody is simply those of Fab and Fc superimposed. It has also been shown that site specific assignments of carbonyl resonances can be made by means of a double labeling technique developed by Kainosho and coworkers (Biochemistry 21, 6273–6279 (1982)).This publication has 10 references indexed in Scilit:
- A 1H NMR Method for the Analysis of Antigen-Antibody Interactions: Binding of a Peptide Fragment of Lysozyme to Anti-Lysozyme Monoclonal AntibodyThe Journal of Biochemistry, 1987
- Local structural features around the C-terminal segment of Streptomyces subtilisin inhibitor studied by the carbonyl carbon nuclear magnetic resonances of three phenylalanyl residuesBiochemistry, 1987
- Proton nuclear magnetic resonance study on the dynamics of the conformation of the hinge segment of human G1 immunoglobulinBiochemistry, 1985
- Proton nuclear magnetic resonance study of human immunoglobulins G1 and their proteolytic fragments: structure of the hinge region and effects of a hinge region deletion on internal flexibilityBiochemistry, 1985
- 1H NMR studies of the Fc region of human IgG1 and IgG3 immunoglobulins: Assignment of histidine resonances in the CH3 domain and identification of IgG3 protein carrying G3m(st) allotypesMolecular Immunology, 1983
- Assignment of the three methionyl carbonyl carbon resonances in Streptomyces subtilisin inhibitor by a carbon-13 and nitrogen-15 double-labeling technique. A new strategy for structural studies of proteins in solutionBiochemistry, 1982
- Rapid isolation of cloned isotype switch variants using fluorescence activated cell sortingCytometry, 1982
- Proton nuclear magnetic resonance studies of human immunoglobulins: conformation of the hinge region of the IgG1 immunoglobulinBiochemistry, 1980
- Proton nuclear magnetic resonance studies of human immunoglobulins. Solution conformation of the constant domain of the .lambda. light chains and identification of the isotypesBiochemistry, 1980
- Proton nuclear magnetic resonance studies of Bence-Jones proteinsBiochemistry, 1979