4-h Identification of Pseudomonas aeruginosa with 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan

Publisher Website
Google Scholar
Abstract
Gram-negative bacilli (361) were evaluated for their ability to grow in the presence of 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (CDP). The minimum time required for the production of visual turbidity in brain heart infusion broth was 4 h in shake cultures at 35.degree. C. The minimal inhibitory concentration (MIC) of CDP in brain heart infusion broth was determined for 174 isolates. The MIC for all 40 P. aeruginosa isolates tested were > 50 .mu.g/ml. The MIC for the other 53 pseudomonads tested were .ltoreq. 5 .mu.g/ml. Among 81 other gram-negative bacilli tested, the MIC for 4 were 15 .mu.g/ml, the MIC for 15 were 10 .mu.g/ml, the MIC for 21 were 5 .mu.g/ml and the MIC for 41 were .ltoreq. 1 .mu.g/ml. Thus, 361 gram-negative bacilli were inoculated into brain heart infusion broth containing 15 .mu.g of CDP/ml and incubated on a shaker for 4 h. The only bacteria that produced visual turbidity were identified as P. aeruginosa (170 of 170 isolates).