A COMPARISON OF SOUTHERN BLOT AND POLYMERASE CHAIN REACTION AS TECHNIQUES TO IDENTIFY LOSS OF DNA HETEROZYGOSITY IN COLORECTAL CANCER
- 1 August 1994
- journal article
- research article
- Published by Wiley in Anz Journal of Surgery
- Vol. 64 (8) , 556-557
- https://doi.org/10.1111/j.1445-2197.1994.tb02285.x
Abstract
Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100% concordance rate in normal tissue but a 90.9% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.Keywords
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