Simultaneous Determination of Seven Compounds in Snow Lotus Herb Using High-Performance Liquid Chromatography

1 May 2003

journal article
Published by Oxford University Press (OUP) in Journal of Chromatographic Science

Vol. 41 (5) , 241-244
https://doi.org/10.1093/chromsci/41.5.241

Abstract

A reversed-phase high-performance liquid chromatographic method is developed for the quantitative determination of seven components of Snow Lotus Herb (Saussurea tridactyla Sch.-Bip.ex Hook.f.): umbelliferonglucoside, luteolin-7-O-beta-D-glucoside, rutin, apigenin-7-O-beta-D-glucoside, kaempferol-3-O-beta-D-glucoside, apigenin-7-O-beta-D-rutinoside, and luteolin. Samples are analyzed by means of a reverse-phase column (Diamonsil C(18)) using methanol and water under gradient conditions as the mobile phase for 60 min. This method offers selectivity, accuracy, precision, linearity, and ruggedness, as well as efficiency and ease.

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