CHARACTERIZATION OF THE CYTO-TOXIN PRODUCED BY MACROPHAGES IN RESPONSE TO DENGUE VIRUS-INDUCED CYTO-TOXIC FACTOR

  • 1 January 1983
    • journal article
    • research article
    • Vol. 64  (2) , 185-190
Abstract
Dengue type 2 virus-induced cytotoxic factor (CF) induces macrophages to produce a cytotoxin (CF2) which kills mainly the macrophages, some of the T lymphocytes and has no effect on B-lymphocytes of normal mouse spleen. The findings of the present study show that CF2 is heat-labile, trypsin-sensitive and unstable at acid and alkaline pH. It is a low MW product, as it is dialyzable, non-sedimentable on ultracentrifugation at 103,500 g for 3 h and passes through 0.22 .mu.m Millipore filter. It is absorbed onto the target normal mouse spleen cells. The properties of CF and CF2 have been compared.