Ozone-induced metabolic changes in Scots pine: mRNA isolation and analysis of in vitro translated proteins

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Abstract
A rapid isolation procedure for intact poly(A)+ RNA from Scots pine (Pinussylvestris L.) is described. Translation products of poly(A)+ RNA isolated from 6-week-old Scots pine seedlings treated with ozone (0.3 μL/L) for 8 h were analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Both qualitative and quantitative differences in the translation products were observed after ozone fumigation. Expression of specific mRNAs, coding for stress-induced polypeptides between 12 kD and 50 kD, was demonstrated after 8 h of ozone treatment.

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