Wound-Tumor

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Abstract
Tumors incited on the roots of the sweet clover Rumex acetosa by the wound-tumor virus were isolated and grown on a synthetic medium. The tumor tissue has a high phosphate requirement and liberates an alpha-amylase into the medium in which it grows. Normal tissues grow very poorly or not at all in such a medium and do not liberate detectable amounts of alpha-amylase. Virus maintained in sweet clover for many years without access to the insect vector (a few species of closely related leafhoppers) loses its ability to be transmitted by the insect. This vectorless virus is less virulent than the wild or vectorial virus. Wound-tumor in plants resembles virus tumors in animals not only because of the virus etiology but also because of the important influence of the heredity of the host on tumor expression.

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